Modifying Enzymes
New England Biolabs, Inc. RtcB Ligase – 25 reactions
RtcB Ligase from E. coli joins single stranded RNA with a 3' -phosphate or 2' ,3' -cyclic phosphate to another RNA with a 5' -hydroxyl. Ligation requires both GTP and MnCl2 and proceeds through a 3' -guanylate intermediate . For substrates with a 2' ,3' -cyclic phosphate end, hydrolysis to a 3' -phosphate precedes 3' end activation with GMP and ligation.
Abcam Trovaflo x acin mesylate, Fluoroquinolone antibiotic agent, 10MG
MW 512.46 g/mol, Purity >98%. Fluoroquinolone antibiotic agent. Topoisomerase IV and DNA gyrase inhibitor. Reversibly inhibits DNA synthesis. Inhibits PANX1 (IC₅₀ = 4 μM). Shows antibiotic effects in vivo (MIC₉₀ = 244 nM, pneumococci).
The product is subject to the following: Abcam Restricted Use Statement
Creative Biomart Native Bacillus licheniformis Protease
AbbrProtease, Native (Bacillus licheniformis)AliasProteaseSourceBacillus licheniformisApplicationsThe product has been used with other enzymes for in situ proteolysis to produce crystals suitable for structure determination. It has also been used in the process of isolation of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria that can be used for functional in vitro studies. This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions.Product OverviewThe enzyme is found to be stable at pH 8-10, retaining activity of up to 90% for 24 hours. It shows maximum activity at temperatures between 55-60°C.FormType VIII, lyophilized powder; Type I, aqueous solutionEnzyme Commission NumberEC 3.4.21.62
Chem-Impex International, Inc. Pepsin 1:10000 | 9001-75-6 | MFCD00081840 | 100G
Pepsin 1:10000, 9001-75-6, MFCD00081840, 100G
AVIDITY LLC BIRA500-RT Biotin Protein Ligase, 2 vials of 20 μL each, 40 μg total, Lyophilized reaction kit, Covalent addition of d-biotin via ATP, >99% purity
The BIRA500-RT Biotin Protein Ligase is a lyophilized reaction kit designed for efficient and targeted biotinylation. This process is crucial in various applications such as affinity chromatography, protein immobilization, and detection with anti-biotin antibodies or avidin conjugates. Optimized for use with AviTag™ peptides, the kit enhances reaction rates, reduces enzyme use, and shortens incubation times, minimizing issues related to proteases and protein instability.
- Enzyme name: BirA biotin-protein ligase
- EC number: 6.3.4.15
- Form: Lyophilized reaction kit
- Source: E. coli wild-type
- Purity: >99%
- Molecular weight: 33,500 Daltons
- Activity: >8000 Units/μg of BirA
- Recommended substrate: AviTag™ peptide sequences
- Stored lyophilized: 1 year at 4°C
- Reconstituted storage: 2 weeks at 4°C, long term at -80°C
- Price: $250.00
Bioworld Macerozyme R-10, 5 g
Macerozyme R-10Macerating enzyme from Rhizopus sp. suitable for the isolation of plant cells and can be used in combination with cellulase.Specification:Used in plant protoplast preparation to digest cell wall prior to oranelle isolation.A multi-component enzyme mixture containing approximately: * Cellulase: 0.1 U/mg * Hemicellulase: 0.25 U/mg * Pectinase: 0.5 U/mgSource: Rhizopus sp.Solubility: Soluble in water
Bioworld Macerozyme R-10, 1 g
Macerozyme R-10Macerating enzyme from Rhizopus sp. suitable for the isolation of plant cells and can be used in combination with cellulase.Specification:Used in plant protoplast preparation to digest cell wall prior to oranelle isolation.A multi-component enzyme mixture containing approximately: * Cellulase: 0.1 U/mg * Hemicellulase: 0.25 U/mg * Pectinase: 0.5 U/mgSource: Rhizopus sp.Solubility: Soluble in water
Bioworld alpha-Chymotrypsin, 500 mg
Specification:Grade: USP gradeForm: White crystalline powderChymotrypsin acitvity (dry basis): 1000 U/mgTrypsin: 1%Residue after ignition: 2.5%Loss on drying: 5%Solubility (water, 10,000 U/ml): Pass
Bioworld alpha-Chymotrypsin, 1 g
Specification:Grade: USP gradeForm: White crystalline powderChymotrypsin acitvity (dry basis): 1000 U/mgTrypsin: 1%Residue after ignition: 2.5%Loss on drying: 5%Solubility (water, 10,000 U/ml): Pass
Bioworld Lysozyme, 5 g
Lysozyme Enzyme used in purification protocols of genomic and plasmid DNA to lyse bacterial cells.Ultra Pure GradeSource: Hen Egg White (also called Hen Egg Lysozyme or HEL.)Activity(25 C): >20,000U/mgAlbumin: None detectedSalmonella: None detected
Bioworld Lysozyme, 10 g
Lysozyme Enzyme used in purification protocols of genomic and plasmid DNA to lyse bacterial cells.Ultra Pure GradeSource: Hen Egg White (also called Hen Egg Lysozyme or HEL.)Activity(25 C): >20,000U/mgAlbumin: None detectedSalmonella: None detected